研究方向：

　　　　一、奶牛健康养殖与牛奶品质调控

　　以奶牛健康养殖和牛奶品质提升为核心，围绕“健康瘤胃-健康奶牛-优质牛奶”科学问题，利用现代组学和分子生物学方法，研究日粮营养素与瘤胃微生物的互作关系，阐明瘤胃微生物代谢碳氮的机制及其在牛奶品质形成中的调控机理，揭示营养素调控乳腺细胞合成乳蛋白的信号通路机制。

　　　　二、牛奶质量安全风险评估与营养功能评价

　　开发牛奶质量安全高通量、高灵敏度或快速检测方法，分析牛奶质量安全风险因子的时空分布和转移转化规律，建立牛奶质量安全控制标准与规范。研究牛奶活性因子的变化规律，通过人体细胞等体外模型及动物体内模型，研究牛奶营养因子对细胞或动物生长、发育和健康的影响，建立牛奶品质评价技术体系。

　　代表性成果：

　　1、Luo, C.; Zheng, N.; Zhao, S.; Wang, J. Sestrin2 Negatively Regulates Casein Synthesis through the SH3BP4-mTORC1 Pathway in Response to AA Depletion or Supplementation in Cow Mammary Epithelial Cells. Journal of agricultural and food chemistry 2019, 67 , 4849-4859, doi:10.1021/acs.jafc.9b00716.

　　2、Luo, C.; Zhao, S.; Dai, W.; Zheng, N.; Wang, J. Proteomic Analysis of Lysosomal Membrane Proteins in Bovine Mammary Epithelial Cells Illuminates Potential Novel Lysosome Functions in Lactation. Journal of agricultural and food chemistry 2018, 66 , 13041–13049, doi:10.1021/acs.jafc.8b04508.

　　3、Zhao, S.; Li, G.; Zheng, N.; Wang, J.; Yu, Z. Steam explosion enhances digestibility and fermentation of corn stover by facilitating ruminal microbial colonization. Bioresour Technol 2018, 253 , 244-251, doi:10.1016/j.biortech.2018.01.024.

　　4、Jin, D.; Zhao, S.; Zheng, N.; Bu, D.; Beckers, Y.; Denman, S.E.; McSweeney, C.S.; Wang, J. Differences in Ureolytic Bacterial Composition between the Rumen Digesta and Rumen Wall Based on ureC Gene Classification. Frontiers in Microbiology 2017, 8 , 385, doi:10.3389/fmicb.2017.00385.

　　5、Tian, H.; Wang, W.; Zheng, N.; Cheng, J.; Li, S.; Zhang, Y.; Wang, J. Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows. Journal of proteomics 2015, 125 , 17-28, doi:10.1016/j.jprot.2015.04.014.

　　6、Guo, X.; Wen, F.; Zheng, N.; Luo, Q.; Wang, H.; Wang, H.; Li, S.; Wang, J. Development of an ultrasensitive aptasensor for the detection of aflatoxin B1. Biosensors & bioelectronics 2014, 56 , 340-344, doi:10.1016/j.bios.2014.01.045.

　　7、Zhou, X.; Zheng, N.; Su, C.; Wang, J.; Soyeurt, H. Relationships between Pb, As, Cr, and Cd in individual cows' milk and milk composition and heavy metal contents in water, silage, and soil. Environmental pollution 2019, 255 , 113322, doi:10.1016/j.envpol.2019.113322.

　　8、Wu, C.; Gao, Y.; Li, S.; Huang, X.; Bao, X.; Wang, J.; Zheng, N. Modulation of intestinal epithelial permeability and mucin mRNA (MUC2, MUC5AC, and MUC5B) expression and protein secretion in Caco-2/HT29-MTX co-cultures exposed to aflatoxin M1, ochratoxin A, and zearalenone individually or collectively. Toxicology letters 2019, 309 , 1-9, doi:10.1016/j.toxlet.2019.03.010.

　　9、Li, H.; Wang, Y.; Yang, H.; Liu, L.; Wang, J.; Zheng, N. Lactoferrin Induces the Synthesis of Vitamin B6 and Protects HUVEC Functions by Activating PDXP and the PI3K/AKT/ERK1/2 Pathway. International journal of molecular sciences 2019, 20 , 587, doi:10.3390/ijms20030587.

　　10、Meng, L.; Zhang, Y.; Liu, H.; Zhao, S.; Wang, J.; Zheng, N. Characterization of Pseudomonas spp. and Associated Proteolytic Properties in Raw Milk Stored at Low Temperatures. Frontiers in Microbiology 2017, 8 , 2158, doi:10.3389/fmicb.2017.02158.

　　11、Chen, L.; Wen, F.; Li, M.; Guo, X.; Li, S.; Zheng, N.; Wang, J. A simple aptamer-based fluorescent assay for the detection of Aflatoxin B1 in infant rice cereal. Food chemistry 2017, 215 , 377-382, doi:10.1016/j.foodchem.2016.07.148.

　　12、Yang, Y.; Zheng, N.; Wang, W.; Zhao, X.; Zhang, Y.; Han, R.; Ma, L.; Zhao, S.; Li, S.; Guo, T., et al. N-glycosylation proteomic characterization and cross-species comparison of milk fat globule membrane proteins from mammals. Proteomics 2016, 16 , 2792-2800, doi:10.1002/pmic.201500361.

　　创新团队成员：

团队首席：王加启  执行首席：郑楠

骨干：张养东、赵圣国

助理：刘慧敏、孟璐