一、研究方向

　　1、猪基因工程与材料创制

　　筛选、鉴定与生长、抗病、繁殖、疾病模型等相关的基因，创制目标性状突出、遗传稳定的农用、医用基因工程猪新材料，经过安全评价和生产性能评估，制备抗病、优质等基因工程猪群体。建立相关的安全监测和育种体系的标准。

　　2、猪能量代谢基因挖掘与种质创新

　　筛选、鉴定调控能量代谢关键基因和表观因子，优化基因组定点修饰等猪基因工程技术，获得表型明显的猪新材料或新品系，应用于猪生产及生物医学研究。

　　3、动物功能基因研究及开发

　　应用功能基因组学、蛋白质组学等方法对重要动物性状功能基因进行研究，揭示分子调控机制。MSTN编辑梅山猪的研发。建立高效安全的精原干细胞受体制备技术体系。

　　二、代表性成果

　　1、Cong Tao, Yajun Wang, Ying Zhao, Jianfei Pan, Yiping Fan, Xiaojuan Liang, Chunwei Cao, Jianguo Zhao, Michael J. Petris, Kui Li, Yanfang Wang*. Adipocyte-specific disruption of atpase copper transporting α in mice accelerates lipoatrophy. Diabetologia . 2019 Online. https://doi.org/10.1007/s00125-019-4966-2

　　2、Ying Liu, Yalan Yang, Wentong Li, Hong Ao, Yanmin Zhang, Rong Zhou*, Kui Li*. Effects of melatonin on the synthesis of estradiol and gene expression in pig granulosa cells. Journal of Pineal Research . 2019, 66(2): e12546.

　　3、Yinghui Wei, Qian Gao, Pengxia Niu, Kui Xu, Yiqing Qiu, Yanqing Hu, Shasha Liu, Xue Zhang, Miaoying Yu, Zhiguo Liu, Bingyuan Wang, Yulian Mu*, Kui Li*. Integrative proteomic and phosphoproteomic profiling of testis from wip1 phosphatase-knockout mice: insights into mechanisms of reduced fertility. Molecular & Cellular Proteomics , 2019, 18(2): 216-230.

　　4、Yiting Tang, Bing Pan, Xin Zhou, Kai Xiong, Qian Gao, Lei Huang, Ying Xia, Ming Shen, Shulin Yang, Honglin Liu, Tao Tan, Jianjie Ma, Xuehong Xu, Yulian Mu*, Kui Li*.  Wip1-dependent modulation of macrophage migration and phagocytosis. Redox biology , 2017, 13: 665-673.

　　5、Guoming Liang, Yalan Yang, Guanglin Niu, Zhonglin Tang*, Kui Li*. Genome-wide profiling of Sus scrofa circular RNAs across nine organs and three developmental stages. DNA research , 2017, 24(5): 523-535.

　　6、Zhonglin Tang, Yalan Yang, Zishuai Wang, Shuanping Zhao, Yulian Mu, Kui Li*. Integrated analysis of mirna and mrna paired expression profiling of prenatal skeletal muscle development in three genotype pigs. Scientific Reports, 2015, 5: 15544.

　　7、Jinxue Ruan, Hegang Li, Kui Xu, Tianwen Wu, Jingliang Wei, Rong Zhou, Zhiguo Liu, Yulian Mu, Shulin Yang, Hongsheng Ouyang, Ruby Yanru Chen-Tsai*, Kui Li*. Highly efficient crispr/cas9-mediated transgene knockin at the h11 locus in pigs. Scientific Reports, 2015, 5: 14253.

　　8、Weimin Zhao, Yulian Mu, Lei Ma, Chen Wang, Zhonglin Tang, Shulin Yang, Rong Zhou, Xiaoju Hu, Menghua Li*, Kui Li*. Systematic identification and characterization of long intergenic non-coding rnas in fetal porcine skeletal muscle development. Scientific Reports, 2015, 5: 8957.

　　9、Lei Huang, Chenguang Niu, Belinda Willard, Weimin Zhao, Lan Liu, Wei He, Tianwen Wu, Shulin Yang, Shutang Feng, Yulian Mu*, Lemin Zheng*, Kui Li*. Proteomic analysis of porcine mesenchymal stem cells derived from bone marrow and umbilical cord: implication of the proteins involved in the higher migration capability of bone marrow mesenchymal stem cells. Stem Cell Research & Therapy, 2015, 6(1): 77.

　　10、Zhonglin Tang, Ruyi Liang, Shuanping Zhao, Ruiqi Wang, Ruihua Huang*, Kui Li*.  Cnn3 is regulated by microrna-1 during muscle development in pigs. International Journal of Biological Sciences, 2014, 10(4): 377-385.

　　11、Chaoqun Wang, Shulin Yang, Ningbo Zhang, Yulian Mu, Hongyan Ren, Yefu Wang*, Kui Li*. Long-term supranutritional supplementation with selenate decreases hyperglycemia and promotes fatty liver degeneration by inducing hyperinsulinemia in diabetic db/db mice. PLOS ONE, 2014, 9(7): e101315.

　　12、Yanan Wang, Zhonglin Tang, Yaqi Sun, Hongyang Wang, Chao Wang, Shaobo Yu, Jing Liu, Yu Zhang, Bin Fan, Kui Li*, Bang Liu*. Analysis of genome-wide copy number variations in chinese indigenous and western pig breeds by 60 k snp genotyping arrays. PLoS ONE, 2014, 9(9): e106780.

　　13、Lili Qian, Maoxue Tang, Jinzeng Yang, Qingqing Wang, Chunbo Cai, Shengwang Jiang, Hegang Li, Ke Jiang, Pengfei Gao, Dezun Ma, Yaoxing Chen, Xiaorong An, Kui Li, Wentao Cui*.  Targeted mutations in myostatin by zinc-finger nucleases result in double-muscled phenotype in meishan pigs. Scientific Reports, 2015, 5: 14435.

　　14、Zhonglin Tang, Yong Li, Ping Wan, Xiaoping Li, Shuhong Zhao, Bang Liu, Bin Fan, Mengjin Zhu, Mei Yu, Kui Li*.  Longsage analysis of skeletal muscle at three prenatal stages in tongcheng and landrace pigs. Genome biology, 2007, 8(6): R115.

　　15、李奎, 周荣, 刘志国, 吴添文, 阮进学, 宋春雷, 陶晨雨, 鞠辉明, 樊俊华, 李秋艳, 毕延震, 宋成义, 牟玉莲, 杨述林, 白立景, 李训碧, 郑新民, 张涌, 连正兴, 戴蕴平. 动物基因组编辑. 北京: 科学出版社. 2017.

　　三、创新团队成员

团队首席：王彦芳

骨干：杨述林、王栋、牟玉莲、陶聪、周荣、敖红、崔文涛、刘志国、庞云渭

助理：吴添文、王霄、林育